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Reporter
Part:BBa_K2176003:Design
Designed by: Miranda Halle Group: iGEM16_UChicago (2016-10-14)
yeGFP Reporter for LexA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 162
Illegal BamHI site found at 148
Illegal BamHI site found at 1675
Illegal XhoI site found at 169 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1067
Design Notes
Will add later
Source
We synthesized this part in G-blocks and assembled it via Gibson assembly.