Reporter

Part:BBa_K2176003:Design

Designed by: Miranda Halle   Group: iGEM16_UChicago   (2016-10-14)


yeGFP Reporter for LexA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 162
    Illegal BamHI site found at 148
    Illegal BamHI site found at 1675
    Illegal XhoI site found at 169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1067


Design Notes

Will add later


Source

We synthesized this part in G-blocks and assembled it via Gibson assembly.

References